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Addgene

pBS2ndd
(Plasmid #97353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescriptII SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2990
  • Total vector size (bp) 3446
  • Vector type
    Bacterial Expression ; cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Growth medium may contain glucose but not beta-D-galactopyranoside such as IPTG or lactose
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nucleoid Disruptive Domain
  • Alt name
    ndd
  • Species
    bacteriophage T4
  • Insert Size (bp)
    456
  • Mutation
    changed methionine 1 to isoleucine
  • GenBank ID
    HM137666
  • Promoter E. coli lacZ promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer GCTCTAGAACTAGTGGATC
  • 3′ sequencing primer CGAGGTCGACGGTATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS2ndd was a gift from Jamorn Somana (Addgene plasmid # 97353 ; http://n2t.net/addgene:97353 ; RRID:Addgene_97353)
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