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Addgene

TetO-Ascl1-puro
(Plasmid #97329)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97329 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Teto-FUW
  • Backbone size w/o insert (bp) 7776
  • Total vector size (bp) 9762
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ascl1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1359
  • GenBank ID
    NM_008553.4
  • Entrez Gene
    Ascl1 (a.k.a. ASH1, Mash1, bHLHa46)
  • Promoter TET-ON

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetO-Ascl1-puro was a gift from Marius Wernig (Addgene plasmid # 97329 ; http://n2t.net/addgene:97329 ; RRID:Addgene_97329)

  • For your References section:

    Generation of pure GABAergic neurons by transcription factor programming. Yang N, Chanda S, Marro S, Ng YH, Janas JA, Haag D, Ang CE, Tang Y, Flores Q, Mall M, Wapinski O, Li M, Ahlenius H, Rubenstein JL, Chang HY, Buylla AA, Sudhof TC, Wernig M. Nat Methods. 2017 May 15. doi: 10.1038/nmeth.4291. 10.1038/nmeth.4291 PubMed 28504679
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