pMSP1D1Q1
(Plasmid
#97326)
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PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97326 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET 28a
- Backbone size w/o insert (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMSP1D1Q1
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Alt nameD1Q1
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SpeciesSynthetic
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Insert Size (bp)639
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MutationAddition of Q residue after T25
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis - TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site Hind III (not destroyed)
- 5′ sequencing primer T7 Pro
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byStephen Sligar, https://www.addgene.org/20061/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSP1D1Q1 was a gift from Michael Marty (Addgene plasmid # 97326 ; http://n2t.net/addgene:97326 ; RRID:Addgene_97326)
