Desmin/pBSSK+
(Plasmid
#97207)
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PurposeDesmin (NM_001927, nt 258 to 395) in pBSSK+
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBluescript SK (+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 3197
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDesmin
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SpeciesH. sapiens (human)
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Insert Size (bp)234
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GenBank IDNM_001927
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Entrez GeneDES (a.k.a. CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Desmin/pBSSK+ was a gift from Rita Shiang (Addgene plasmid # 97207 ; http://n2t.net/addgene:97207 ; RRID:Addgene_97207) -
For your References section:
A schizophrenia associated CMYA5 allele displays differential binding with desmin. Hsiung A, Naya FJ, Chen X, Shiang R. J Psychiatr Res. 2019 Apr;111:8-15. doi: 10.1016/j.jpsychires.2019.01.007. Epub 2019 Jan 10. 10.1016/j.jpsychires.2019.01.007 PubMed 30658136