pcDNA3-CARMA2cl-HA
(Plasmid
#97195)
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PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARD14 isoform 2
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Alt nameCARMA2cl
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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Mutationnone
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GenBank IDNM_052819
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Entrez GeneCARD14 (a.k.a. BIMP2, CARMA2, PRP, PSORS2, PSS1)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results found P363R within the CARD14 translation compared to NM_052819 (NP_438170.1)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-CARMA2cl-HA was a gift from Pasquale Vito (Addgene plasmid # 97195 ; http://n2t.net/addgene:97195 ; RRID:Addgene_97195) -
For your References section:
Alternative splicing of CARMA2/CARD14 transcripts generates protein variants with differential effect on NF-kappaB activation and endoplasmic reticulum stress-induced cell death. Scudiero I, Zotti T, Ferravante A, Vessichelli M, Vito P, Stilo R. J Cell Physiol. 2011 Dec;226(12):3121-31. doi: 10.1002/jcp.22667. 10.1002/jcp.22667 PubMed 21302310