pBS-AMVpl-γWT-nAchR
(Plasmid
#97187)
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PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97187 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2964
- Total vector size (bp) 4743
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Modifications to backboneNA
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGamma nicotinic acetylcholine receptor
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Alt nameγWTnAchR
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SpeciesM. musculus (mouse)
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Insert Size (bp)1560
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MutationWT
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GenBank IDP04760.1
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Entrez GeneChrng (a.k.a. Achr-3, Acrg)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bcl1 (unknown if destroyed)
- 3′ cloning site Xba1 (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer pBSKSreverse(+) primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byLester Lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-AMVpl-γWT-nAchR was a gift from Henry Lester (Addgene plasmid # 97187 ; http://n2t.net/addgene:97187 ; RRID:Addgene_97187) -
For your References section:
Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Labarca C, Nowak MW, Zhang H, Tang L, Deshpande P, Lester HA. Nature. 1995 Aug 10. 376(6540):514-6. 10.1038/376514a0 PubMed 7637783