pFUSE-DEST_R1R4
(Plasmid #97186)

• Purpose: (Empty Backbone) For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector
• Depositing Lab: Kenneth Scott
• Publication: Lu et al Cancer Res. 2017 May 16. pii: canres.2745.2016. doi: 10.1158/0008-5472.CAN-16-2745.

Backbone

• Vector backbone: pFUSE-DEST_R1R4
• Backbone size (bp): 10044
• Vector type: Mammalian Expression, Lentiviral ; Gateway
• Promoter: EF1 alpha
• Selectable markers: Puromycin
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EF1a For
• 3′ sequencing primer: V5 Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Recombine Gene A + Gene B into this Destination vector by Gateway LR reaction to make the final fusion.

The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017).