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pSi-check2-hRac2 - mut 3'UTR
(Plasmid #97161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Psi-check2
  • Backbone size w/o insert (bp) 6250
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAC 3'UTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    780
  • Mutation
    miR-722 Binding Mutant
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

infusion cloning was used

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSi-check2-hRac2 - mut 3'UTR was a gift from Qing Deng (Addgene plasmid # 97161 ; http://n2t.net/addgene:97161 ; RRID:Addgene_97161)

  • For your References section:

    Overexpression of microRNA-722 fine-tunes neutrophilic inflammation through inhibiting Rac2 in zebrafish. Hsu AY, Wang D, Gurol T, Zhou W, Zhu X, Lu HY, Deng Q. Dis Model Mech. 2017 Sep 27. pii: dmm.030791. doi: 10.1242/dmm.030791. 10.1242/dmm.030791 PubMed 28954734
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