miR-vector-RFP pCDNA
(Plasmid
#97143)
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Purpose(Empty Backbone) for expression of miRNA and TagRFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 97143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCDNA 3.1
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Backbone manufacturerXiaokun Shu (Addgene plasmid # 73176)
- Backbone size (bp) 5300
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- TagRFP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- TagRFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR-vector-RFP pCDNA was a gift from Qing Deng (Addgene plasmid # 97143 ; http://n2t.net/addgene:97143 ; RRID:Addgene_97143) -
For your References section:
MicroRNA-223 Suppresses the Canonical NF-kappaB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation. Zhou W, Pal AS, Hsu AY, Gurol T, Zhu X, Wirbisky-Hershberger SE, Freeman JL, Kasinski AL, Deng Q. Cell Rep. 2018 Feb 13;22(7):1810-1823. doi: 10.1016/j.celrep.2018.01.058. 10.1016/j.celrep.2018.01.058 PubMed 29444433
