miR-722 plix4.03
(Plasmid
#97140)
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PurposeLentiviral tet-inducible expression of miR-722and Dendra 2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneplix 4.03
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Backbone manufacturerDavid Root (Addgene plasmid # 41395)
- Backbone size w/o insert (bp) 7700
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemiR-722
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1500
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Entrez Genemir722 (a.k.a. dre-mir-722)
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Tag
/ Fusion Protein
- dendra2 (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
infusion cloning was used
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR-722 plix4.03 was a gift from Qing Deng (Addgene plasmid # 97140 ; http://n2t.net/addgene:97140 ; RRID:Addgene_97140) -
For your References section:
Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q. Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. 10.1073/pnas.1905221116 PubMed 31451657