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Addgene

miR-722 plix4.03
(Plasmid #97140)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97140 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    plix 4.03
  • Backbone manufacturer
    David Root (Addgene plasmid # 41395)
  • Backbone size w/o insert (bp) 7700
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-722
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1500
  • Entrez Gene
    mir722 (a.k.a. dre-mir-722)
  • Tag / Fusion Protein
    • dendra2 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

infusion cloning was used

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miR-722 plix4.03 was a gift from Qing Deng (Addgene plasmid # 97140 ; http://n2t.net/addgene:97140 ; RRID:Addgene_97140)
  • For your References section:

    Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q. Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. 10.1073/pnas.1905221116 PubMed 31451657