P20_992-MxiE
(Plasmid
#97099)
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PurposeExpresses MxiE in the presence of Pseudomonas fluorescence sigma factor ECF20_992
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBEST
- Backbone size w/o insert (bp) 3294
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMxiE
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Insert Size (bp)756
- Promoter P20_992
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe MxiE gene was derived from a plasmid made by Chris Voigt's lab.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P20_992-MxiE was a gift from Richard Murray (Addgene plasmid # 97099 ; http://n2t.net/addgene:97099 ; RRID:Addgene_97099)