pYFP-NEAT1_IS_RT
(Plasmid #97091)

• Purpose: Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
• Depositing Lab: Archa Fox
• Publication: Li et al RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEAT1_IS_RT
• Species: H. sapiens (human)
• Entrez Gene: NEAT1 (a.k.a. LINC00084, NCRNA00084, TP53LC15, TncRNA, VINC)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The entire repair template is synthesized and then cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y. Also see original publication for mutations on the repair template.