pYFP-NEAT1m_v1-RT
(Plasmid #97089)

• Purpose: Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
• Depositing Lab: Archa Fox
• Publication: Li et al RNA. 2017 Mar 21. pii: rna.059477.116. doi: 10.1261/rna.059477.116.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEAT1m_v1-RT
• Species: H. sapiens (human)
• Entrez Gene: NEAT1 (a.k.a. LINC00084, NCRNA00084, TP53LC15, TncRNA, VINC)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The left arm of the repair template was cloned using the AseI site. The right arm was cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y