pBS-AMVpl-β1WT-nAchR
(Plasmid #97080)

• Purpose: To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
• Depositing Lab: Henry Lester
• Publication: Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Backbone

• Vector backbone: pBluescript KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2964
• Total vector size (bp): 4689
• Modifications to backbone: NA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beta1 nicotinic acetylcholine receptor
• Alt name: b1WTnAchR
• Species: M. musculus (mouse)
• Insert Size (bp): 1506
• Mutation: WT
• GenBank ID: NM-009601
• Entrez Gene: Chrnb1 (a.k.a. Achr-2, Acrb)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstE 1 (unknown if destroyed)
• 3′ cloning site: BstX1 (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: pBSKSreverse(+) primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Lester Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry