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pcDNA3-CARMA2sh-E142G
(Plasmid #97070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7620
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARD14 isoform 3
  • Alt name
    CARMA2sh
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2220
  • Mutation
    changed Glutamic Acid 142 to Glycine
  • GenBank ID
    EU652409.1 NM_001257970.1
  • Entrez Gene
    CARD14 (a.k.a. BIMP2, CARMA2, PRP, PSORS2, PSS1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-CARMA2sh-E142G was a gift from Pasquale Vito (Addgene plasmid # 97070 ; http://n2t.net/addgene:97070 ; RRID:Addgene_97070)

  • For your References section:

    CARMA2sh and ULK2 control pathogen-associated molecular patterns recognition in human keratinocytes: psoriasis-linked CARMA2sh mutants escape ULK2 censorship. Scudiero I, Mazzone P, D'Andrea LE, Ferravante A, Zotti T, Telesio G, De Rubis G, Reale C, Pizzulo M, Muralitharan S, Vito P, Stilo R. Cell Death Dis. 2017 Feb 23;8(2):e2627. doi: 10.1038/cddis.2017.51. 10.1038/cddis.2017.51 PubMed 28230860
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