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Addgene

pEHISSSO6206
(Plasmid #97065)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEHISTEV
  • Backbone size w/o insert (bp) 5353
  • Total vector size (bp) 5641

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression in DE3 strains
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    S. solfataricus orf SSO6206
  • Alt name
    SSO6206
  • Species
    Sulfolobus solfataricus
  • Insert Size (bp)
    288
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal TEV protease cleavable 6xHis tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEHISSSO6206 was a gift from Huanting Liu & James Naismith (Addgene plasmid # 97065 ; http://n2t.net/addgene:97065 ; RRID:Addgene_97065)

  • For your References section:

    The Scottish Structural Proteomics Facility: targets, methods and outputs. Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, Naismith JH. J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 10.1007/s10969-010-9090-y. Epub 2010 Apr 24. 10.1007/s10969-010-9090-y PubMed 20419351
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