pcDNA5/BirA/FLAG-DDX54
(Plasmid
#97063)
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PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 97063 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5-BirA-FLAG-N-term
- Backbone size w/o insert (bp) 7838
- Total vector size (bp) 8856
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDDX54
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Alt nameDP97
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SpeciesH. sapiens (human)
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Insert Size (bp)2646
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GenBank IDNM_024072.3
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Entrez GeneDDX54 (a.k.a. DP97)
- Promoter tetO2
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Tags
/ Fusion Proteins
- BirA (N terminal on backbone)
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer pcDNA3.1-reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/BirA/FLAG-DDX54 was a gift from Markus Landthaler (Addgene plasmid # 97063 ; http://n2t.net/addgene:97063 ; RRID:Addgene_97063) -
For your References section:
DDX54 regulates transcriptome dynamics during DNA damage response. Milek M, Imami K, Mukherjee N, Bortoli F, Zinnall U, Hazapis O, Trahan C, Oeffinger M, Heyd F, Ohler U, Selbach M, Landthaler M. Genome Res. 2017 Aug;27(8):1344-1359. doi: 10.1101/gr.218438.116. Epub 2017 Jun 8. 10.1101/gr.218438.116 PubMed 28596291