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Addgene

pcDNA5/FRT/TO-DDX54-FLAG/HA
(Plasmid #97062)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Thermo Fischer
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 7832
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX54
  • Alt name
    DP97
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2646
  • GenBank ID
    NM_024072.3
  • Entrez Gene
    DDX54 (a.k.a. DP97)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG/HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer pcDNA3.1-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-DDX54-FLAG/HA was a gift from Markus Landthaler (Addgene plasmid # 97062 ; http://n2t.net/addgene:97062 ; RRID:Addgene_97062)

  • For your References section:

    DDX54 regulates transcriptome dynamics during DNA damage response. Milek M, Imami K, Mukherjee N, Bortoli F, Zinnall U, Hazapis O, Trahan C, Oeffinger M, Heyd F, Ohler U, Selbach M, Landthaler M. Genome Res. 2017 Aug;27(8):1344-1359. doi: 10.1101/gr.218438.116. Epub 2017 Jun 8. 10.1101/gr.218438.116 PubMed 28596291
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