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pEAHISSSO2373
(Plasmid #97058)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEAHISTEV, modified from pDEST14
  • Backbone manufacturer
    BSRC, University of St Andrews
  • Backbone size w/o insert (bp) 3705
  • Total vector size (bp) 4275
  • Modifications to backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protei expression in DE3 strains
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. solfataricus orf SSO2373
  • Alt name
    SSO2373
  • Species
    Sulfolobus solfataricus
  • Insert Size (bp)
    570
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal TEV protease cleavable 6xHis tag (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results found an Q69H mutation in HQPRT. This natural variant sequence is not known to affect enzyme function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEAHISSSO2373 was a gift from Huanting Liu & James Naismith (Addgene plasmid # 97058 ; http://n2t.net/addgene:97058 ; RRID:Addgene_97058)

  • For your References section:

    The Scottish Structural Proteomics Facility: targets, methods and outputs. Oke M, Carter LG, Johnson KA, Liu H, McMahon SA, Yan X, Kerou M, Weikart ND, Kadi N, Sheikh MA, Schmelz S, Dorward M, Zawadzki M, Cozens C, Falconer H, Powers H, Overton IM, van Niekerk CA, Peng X, Patel P, Garrett RA, Prangishvili D, Botting CH, Coote PJ, Dryden DT, Barton GJ, Schwarz-Linek U, Challis GL, Taylor GL, White MF, Naismith JH. J Struct Funct Genomics. 2010 Jun;11(2):167-80. doi: 10.1007/s10969-010-9090-y. Epub 2010 Apr 24. 10.1007/s10969-010-9090-y PubMed 20419351
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