13SA-csy4
(Plasmid #97037)

• Purpose: Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
• Depositing Lab: Chris Jeans
• Publication: Series-371: N- or C-terminal Tag E.coli T7 Expression Vectors with Biobrick Polycistronic Restriction Sites and Csy4 cleavage (unpublished)

Backbone

• Vector backbone: pET co-transformation cloning vector (13S-A)
• Backbone size w/o insert (bp): 3566
• Total vector size (bp): 4135
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cys4
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 564
• Promoter: T7

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Supplemental Documents:
  • 13sa-csy4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry