p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
(Plasmid #97012)

• Purpose: (Empty Backbone) Rosa26 targeting vector for intersectional Cre and FLP responsive cassette
• Depositing Lab: Russell Ray
• Publication: Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0.

Backbone

• Vector backbone: p15a ori vector
• Backbone size (bp): 10620
• Vector type: Mammalian Expression, Cre/Lox, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Resource Information

• Supplemental Documents:
  • p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_PStop_WPRE_bgh_pA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is for ES cell targeting of an intersectional Cre/FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, LoxP-flanked EcoRV cloning site and stop cassette, PmeI cloning site, WPRE and bgh pA elements, and a 3' homology arm. A subtractive cDNA of interest (expressed in response to FLP but not Cre recombination) can be cloned into the EcoRV site. The intersectional cDNA of interest (expressed in response to both Cre and FLP recombination) can be cloned into the PmeI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.