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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA
(Plasmid #97010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 97010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a ori vector
  • Backbone size (bp) 9204
  • Vector type
    Mammalian Expression, Cre/Lox, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is for ES cell targeting of a FLP responsive cassette into the Rosa26 locus. It consists of a 5' homology arm, CAG promoter, FRT-flanked neomycin resistance and stop cassette, SwaI cloning site, WPRE and bgh pA elements, and a 3' homology arm. The cDNA of interest (expressed in response to FLP recombination) can be cloned into the SwaI site. This vector lacks the sgRNA target sequence found in the px330_Rosa_sgRNA plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_FNeoStop_WPRE_bgh_pA was a gift from Russell Ray (Addgene plasmid # 97010 ; http://n2t.net/addgene:97010 ; RRID:Addgene_97010)

  • For your References section:

    A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping. Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, Tolias AS, Costa-Mattioli M, Ray RS. BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0. 10.1186/s12915-022-01227-0 PubMed 35086530
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