pMGF196
(Plasmid #97005)

• Purpose: Expresses LEMD2-mCherry in mammalian cells under a crippled promoter
• Depositing Labs: Adam Frost, Wesley Sundquist
• Publication: Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.

Backbone

• Vector backbone: pCMV
• Total vector size (bp): 8155
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LEMD2
• Alt name: LEM2
• Species: H. sapiens (human)
• Entrez Gene: LEMD2 (a.k.a. CTRCT42, LEM2, NET25, dJ482C21.1)
• Promoter: CMV delta 5
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry