pMDC43-FIT2
(Plasmid #96993)

• Purpose: Express GFP-tagged mouse FIT2 gene in plants.
• Depositing Lab: Kent Chapman
• Publication: Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678.

Backbone

• Vector backbone: pMDC43
• Backbone size w/o insert (bp): 12460
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MmFIT2
• Species: M. musculus (mouse)
• Insert Size (bp): 789
• Mutation: E2D (please see depositor comments below)
• Entrez Gene: Fitm2 (a.k.a. D930001I22Rik, Fit2)
• Promoter: 35S
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: PacI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html

Please note that mutation E2D was found during Addgene's quality control process. The depositor noted that this mutation does NOT affect plasmid function.