pMDC32-FIT2
(Plasmid
#96990)
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PurposeExpress mouse FIT2 gene in plants.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 96990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMDC32
- Backbone size w/o insert (bp) 11752
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMmFIT2
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SpeciesM. musculus (mouse)
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Insert Size (bp)789
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Entrez GeneFitm2 (a.k.a. D930001I22Rik, Fit2)
- Promoter 35S
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site PacI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDC32-FIT2 was a gift from Kent Chapman (Addgene plasmid # 96990 ; http://n2t.net/addgene:96990 ; RRID:Addgene_96990) -
For your References section:
Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants. Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD. Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678. 10.1111/pbi.12678 PubMed 27987528
