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Addgene

pRS315-PGK-SEIPIN1
(Plasmid #96986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315-PGK
  • Backbone size w/o insert (bp) 7840
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEIPIN1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1104
  • Entrez Gene
    AT5G16460 (a.k.a. AT5G16460, MQK4.19, MQK4_19)
  • Promoter Phosphoglycerate Kinase (PGK) promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site PstI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutations V69A and D300N in LEU2 were found during Addgene's quality control process. The depositor noted that these mutations do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS315-PGK-SEIPIN1 was a gift from Kent Chapman (Addgene plasmid # 96986 ; http://n2t.net/addgene:96986 ; RRID:Addgene_96986)

  • For your References section:

    Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation and Influence Lipid Droplet Proliferation. Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD. Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 2015 Sep 11. 10.1105/tpc.15.00588 PubMed 26362606
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