pMDC84-SEIPIN1
(Plasmid #96979)

• Purpose: Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.
• Depositing Lab: Kent Chapman
• Publication: Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 2015 Sep 11.

Backbone

• Vector backbone: pMDC84
• Backbone size w/o insert (bp): 12460
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SEIPIN1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1104
• Entrez Gene: AT5G16460 (a.k.a. AT5G16460, MQK4.19, MQK4_19)
• Promoter: 35S
• Tags / Fusion Proteins:
  • 6xHis (C terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html