Skip to main content
Addgene

pMDC43-SEIPIN3
(Plasmid #96978)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMDC43
  • Backbone size w/o insert (bp) 12460
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEIPIN3
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1530
  • Entrez Gene
    AT2G34380 (a.k.a. AT2G34380, F13P17.31)
  • Promoter 35S
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC43-SEIPIN3 was a gift from Kent Chapman (Addgene plasmid # 96978 ; http://n2t.net/addgene:96978 ; RRID:Addgene_96978)

  • For your References section:

    Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation and Influence Lipid Droplet Proliferation. Cai Y, Goodman JM, Pyc M, Mullen RT, Dyer JM, Chapman KD. Plant Cell. 2015 Sep;27(9):2616-36. doi: 10.1105/tpc.15.00588. Epub 2015 Sep 11. 10.1105/tpc.15.00588 PubMed 26362606
Related items:
Commonly requested with: