pMGF157
(Plasmid #96972)

• Purpose: ish1-mCherry insertion cassette with Hygro resistance gene
• Depositing Labs: Adam Frost, Wesley Sundquist
• Publication: Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.

Backbone

• Vector backbone: pGEM GST vector
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 8500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ish1
• Species: S. pombe (fission yeast)
• Insert Size (bp): 3700
• Mutation: ish1 C-terminal domain fused with mCherry + hygro resistance gene
• Entrez Gene: ish1 (a.k.a. SPBC365.12c)
• Promoter: N/A
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCTGTTCCTAATTGGGCTGC
• 3′ sequencing primer: ctccactaactcgtttccaaca

Resource Information

• Supplemental Documents:
  • pMGF157-pGEM-GST-pp-ish1-linker-mCherry-Hygro-tail.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry