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PurposeDox-inducible expression of Ebf1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 96965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE3G-IRES
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEbf1
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SpeciesM. musculus (mouse)
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Entrez GeneEbf1 (a.k.a. Ebf, O/E-1, OE-1, Olf-1, Olf1)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRE3G-EBF1 was a gift from Elena Cattaneo (Addgene plasmid # 96965 ; http://n2t.net/addgene:96965 ; RRID:Addgene_96965) -
For your References section:
Differentiation of human telencephalic progenitor cells into MSNs by inducible expression of Gsx2 and Ebf1. Faedo A, Laporta A, Segnali A, Galimberti M, Besusso D, Cesana E, Belloli S, Moresco RM, Tropiano M, Fuca E, Wild S, Bosio A, Vercelli AE, Biella G, Cattaneo E. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1234-E1242. doi: 10.1073/pnas.1611473114. Epub 2017 Jan 30. 10.1073/pnas.1611473114 PubMed 28137879