EGFP-PH-dL5**
(Plasmid #96947)

• Purpose: Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation
• Depositing Lab: Marcel Bruchez
• Publication: He et al Nat Methods. 2016 Mar;13(3):263-268. doi: 10.1038/nmeth.3735. Epub 2016 Jan 25.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pleckstrin homology domain
• Alt name: PH domain
• Species: H. sapiens (human)
• Insert Size (bp): 510
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • dL5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind3 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • Annotated Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry