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Addgene

pET28b-BE3∆UGI
(Plasmid #96943)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5334
  • Total vector size (bp) 10203
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BE3∆UGI
  • Alt name
    His6-rAPOBEC1-XTEN-nCas9
  • Species
    R. norvegicus (rat); S. pyogenes
  • Insert Size (bp)
    4869
  • Promoter T7
  • Tag / Fusion Protein
    • His6 tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-BE3∆UGI was a gift from Daesik Kim (Addgene plasmid # 96943 ; http://n2t.net/addgene:96943 ; RRID:Addgene_96943)

  • For your References section:

    Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Kim D, Lim K, Kim ST, Yoon SH, Kim K, Ryu SM, Kim JS. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10. 10.1038/nbt.3852 PubMed 28398345
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