pLenti_CamKIIa_GFP
(Plasmid #96941)

• Purpose: To label mature excitatory iPSC-derived human neurons
• Depositing Labs: Ricardo Dolmetsch, Oleksandr Shcheglovitov
• Publication: Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. doi: 10.1038/nature12618. Epub 2013 Oct 16.

Backbone

• Vector backbone: pLenti
• Total vector size (bp): 9971
• Vector type: Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Green fluorescent protein
• Alt name: GFP
• Promoter: CaMKIIa

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: aCCGGTcgccaccatggtg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry