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Addgene

pMSCVpuro-mTagBFP2
(Plasmid #96935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCVpuro
  • Backbone size w/o insert (bp) 6295
  • Total vector size (bp) 7018
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Monomeric Tag Blue Fluorescent Protein
  • Alt name
    mTagBFP2
  • Species
    Synthetic
  • Insert Size (bp)
    729

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer MSCVpuro 5 primer
  • 3′ sequencing primer MSCVpuro 3 primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Base vector obtained from Robert Hawley.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCVpuro-mTagBFP2 was a gift from Lee Grimes (Addgene plasmid # 96935 ; http://n2t.net/addgene:96935 ; RRID:Addgene_96935)

  • For your References section:

    A guide to choosing fluorescent protein combinations for flow cytometric analysis based on spectral overlap. Kleeman B, Olsson A, Newkold T, Kofron M, DeLay M, Hildeman D, Grimes HL. Cytometry A. 2018 Mar 13. doi: 10.1002/cyto.a.23360. 10.1002/cyto.a.23360 PubMed 29533508
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