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Addgene

XLone-GFP
(Plasmid #96930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 5607
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green Fluorescent Protein
  • Alt name
    GFP
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13 FWD
  • 3′ sequencing primer M13 REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-GFP was a gift from Xiaojun Lian (Addgene plasmid # 96930 ; http://n2t.net/addgene:96930 ; RRID:Addgene_96930)

  • For your References section:

    An all-in-one, Tet-On 3G inducible PiggyBac system for human pluripotent stem cells and derivatives. Randolph LN, Bao X, Zhou C, Lian X. Sci Rep. 2017 May 8;7(1):1549. doi: 10.1038/s41598-017-01684-6. 10.1038/s41598-017-01684-6 [pii] PubMed 28484230
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