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Addgene

FLAG-Atoh1 S325A
(Plasmid #96926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atoh1
  • Species
    M. musculus (mouse)
  • Mutation
    Serine 325 changed to Alanine
  • Entrez Gene
    Atoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
  • Tag / Fusion Protein
    • 2xFLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Atoh1 S325A was a gift from Albert Edge (Addgene plasmid # 96926 ; http://n2t.net/addgene:96926 ; RRID:Addgene_96926)

  • For your References section:

    Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development. Cheng YF, Tong M, Edge AS. J Biol Chem. 2016 Sep 30;291(40):21096-21109. Epub 2016 Aug 19. 10.1074/jbc.M116.722124 PubMed 27542412
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