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Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 96918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXPR
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9
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MutationdCas9
- Promoter EF1a
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Tag
/ Fusion Protein
- KRAB (N terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX_311-KRAB-dCas9 was a gift from John Doench & William Hahn & David Root (Addgene plasmid # 96918 ; http://n2t.net/addgene:96918 ; RRID:Addgene_96918) -
For your References section:
Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Rosenbluh J, Xu H, Harrington W, Gill S, Wang X, Vazquez F, Root DE, Tsherniak A, Hahn WC. Nat Commun. 2017 May 23;8:15403. doi: 10.1038/ncomms15403. 10.1038/ncomms15403 PubMed 28534478
