FLAG-Atoh1 del3
(Plasmid
#96913)
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PurposeMurine Atoh1 with amino acids 214-305 removed
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 96913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtoh1
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SpeciesM. musculus (mouse)
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MutationDeletion of amino acids 214-305
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Entrez GeneAtoh1 (a.k.a. Hath1, MATH-1, Math1, bHLHa14)
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Tag
/ Fusion Protein
- 2xFLAG (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Atoh1 del3 was a gift from Albert Edge (Addgene plasmid # 96913 ; http://n2t.net/addgene:96913 ; RRID:Addgene_96913) -
For your References section:
Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development. Cheng YF, Tong M, Edge AS. J Biol Chem. 2016 Sep 30;291(40):21096-21109. Epub 2016 Aug 19. 10.1074/jbc.M116.722124 PubMed 27542412