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Addgene

FlagMBNL1-41
(Plasmid #96906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 HisC
  • Backbone size w/o insert (bp) 5387
  • Total vector size (bp) 6444
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBNL1-41 kDa isoform
  • Species
    H. sapiens (human)
  • Entrez Gene
    MBNL1 (a.k.a. EXP, MBNL)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FlagMBNL1-41 was a gift from Thomas Cooper (Addgene plasmid # 96906 ; http://n2t.net/addgene:96906 ; RRID:Addgene_96906)

  • For your References section:

    Identification of MBNL1 and MBNL3 domains required for splicing activation and repression. Grammatikakis I, Goo YH, Echeverria GV, Cooper TA. Nucleic Acids Res. 2011 Apr;39(7):2769-80. doi: 10.1093/nar/gkq1155. Epub 2010 Nov 24. 10.1093/nar/gkq1155 PubMed 21109529
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