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Addgene

pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)
(Plasmid #96888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 96888 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETduet
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 7634
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Trifunctional enzyme subunit alpha, mitochondrial
  • Alt name
    ECHA
  • Alt name
    Hadha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2214
  • Mutation
    deleted amino acid 1-36, mutated lysine 351 to arginine
  • Entrez Gene
    Hadha (a.k.a. C77020, Mtpa)
  • Promoter T7
  • Tag / Fusion Protein
    • FLAG-tagged (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer forward gene primer
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag) was a gift from Hening Lin (Addgene plasmid # 96888 ; http://n2t.net/addgene:96888 ; RRID:Addgene_96888)

  • For your References section:

    Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function. Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5. 10.1073/pnas.1519858113 PubMed 27051063
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