pET-Duet-ECHA(37-763)-FLAG (no His-tag)
(Plasmid
#96887)
-
Purposeexpresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 96887 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepETduet
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5420
- Total vector size (bp) 7634
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTrifunctional enzyme subunit alpha, mitochondrial
-
Alt nameECHA
-
Alt nameHadha
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)2214
-
Mutationdeleted amino acid 1-36
-
Entrez GeneHadha (a.k.a. C77020, Mtpa)
- Promoter T7
-
Tag
/ Fusion Protein
- FLAG-tagged (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer forward gene primer
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET-Duet-ECHA(37-763)-FLAG (no His-tag) was a gift from Hening Lin (Addgene plasmid # 96887 ; http://n2t.net/addgene:96887 ; RRID:Addgene_96887) -
For your References section:
Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function. Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5. 10.1073/pnas.1519858113 PubMed 27051063