pcDNA5/FRT/TO-AT1R-APEX
(Plasmid
#96847)
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PurposeFor expression of AT1R-APEX in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 96847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerThermo Fisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAT1R-APEX
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SpeciesH. sapiens (human)
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Entrez GeneAGTR1 (a.k.a. AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/FRT/TO-AT1R-APEX was a gift from Andrew Kruse (Addgene plasmid # 96847 ; http://n2t.net/addgene:96847 ; RRID:Addgene_96847) -
For your References section:
Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling. Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC. Cell. 2017 Apr 6;169(2):338-349.e11. doi: 10.1016/j.cell.2017.03.028. 10.1016/j.cell.2017.03.028 PubMed 28388415
