BKK20640
(Bacterial strain
#96119)
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PurposeB subtilis gene deletion of yoqG. Locus tag of deleted gene is BSU20640
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 96119 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneNA
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BKK20640
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Growth instructions7.5 ug/ml kanamycin for agar plate selection. (3ug/ml kanamycin can be used for liquid culture but not necessarily required)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBSU20640
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SpeciesBacillus subtilis 168
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MutationyoqG
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BKK20640 was a gift from Carol Gross (Addgene plasmid # 96119) -
For your References section:
Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis. Koo BM, Kritikos G, Farelli JD, Todor H, Tong K, Kimsey H, Wapinski I, Galardini M, Cabal A, Peters JM, Hachmann AB, Rudner DZ, Allen KN, Typas A, Gross CA. Cell Syst. 2017 Mar 22;4(3):291-305.e7. doi: 10.1016/j.cels.2016.12.013. Epub 2017 Feb 8. 10.1016/j.cels.2016.12.013 PubMed 28189581