BEC00310
(Bacterial strain
#92475)
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PurposeCRISPRi knockdown Genotype: Bacillus subtilis subtilis strain 168, trpC2, lacA::Pxyl-dcas9(ErmR), amyE::Pveg-sgRNA_holB(CmR)
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Bacterial Strain | 92475 | Bacteria in agar stab | 1 | $85 |
Backbone
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Vector backboneNA
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BEC00310
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBSU00310
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SpeciesBacillus subtilis 168
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MutationholB
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
holB; BSU00310; BEC00310
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BEC00310 was a gift from Carol Gross (Addgene plasmid # 92475) -
For your References section:
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. 10.1016/j.cell.2016.05.003 PubMed 27238023