pcDNA3.1+-PAFAH1B1_R22-
(Plasmid #92431)

• Purpose: mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).
• Depositing Labs: Ralf Brandes, Matthias Leisegang
• Publication: Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10.1111/apha.12700.

Backbone

• Vector backbone: pcDNA3.1+
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAFAH1B1
• Species: H. sapiens (human)
• Mutation: R22 (stop codon in position 22)
• Entrez Gene: PAFAH1B1 (a.k.a. LIS1, LIS2, MDCR, MDS, NudF, PAFAH)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry