EcMscL
(Plasmid #92418)

• Purpose: E. coli Mechanosensitive Channel of Large Conductance
• Depositing Lab: Douglas Rees
• Publication: EcMscL (unpublished)

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6137
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MscL
• Alt name: Mechanosensitive Channel of Large Conductance
• Species: E. coli
• Insert Size (bp): 408
• Entrez Gene: mscL (a.k.a. b3291, ECK3277, yhdC)
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 6x Histidine Tag (N terminal on insert)
  • Thrombin Cleavage Site between Histidine Tag and MscL (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • ecmscl-in-pet15b.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry