EcMscL
(Plasmid
#92418)
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PurposeE. coli Mechanosensitive Channel of Large Conductance
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6137
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMscL
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Alt nameMechanosensitive Channel of Large Conductance
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SpeciesE. coli
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Insert Size (bp)408
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Entrez GenemscL (a.k.a. b3291, ECK3277, yhdC)
- Promoter T7 Promoter
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Tags
/ Fusion Proteins
- 6x Histidine Tag (N terminal on insert)
- Thrombin Cleavage Site between Histidine Tag and MscL (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EcMscL was a gift from Douglas Rees (Addgene plasmid # 92418 ; http://n2t.net/addgene:92418 ; RRID:Addgene_92418)
