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Addgene

pDZ2087
(Plasmid #92414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMalC2
  • Backbone manufacturer
    New England Biolabs
  • Total vector size (bp) 7393
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BL21-RIL
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TEV protease
  • Species
    tobacco etch virus
  • Mutation
    L56V/S135G/S219V
  • Promoter tac
  • Tag / Fusion Protein
    • MBP (self cleaving) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer MBP
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BL21-RIL strain is chloramphenicol resistant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ2087 was a gift from David Waugh (Addgene plasmid # 92414 ; http://n2t.net/addgene:92414 ; RRID:Addgene_92414)

  • For your References section:

    Removal of Affinity Tags with TEV Protease. Raran-Kurussi S, Cherry S, Zhang D, Waugh DS. Methods Mol Biol. 2017;1586:221-230. doi: 10.1007/978-1-4939-6887-9_14. 10.1007/978-1-4939-6887-9_14 PubMed 28470608

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