pEHIShqprtR161Q
(Plasmid
#92413)
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PurposehQPRTase harboring R161Q mutation
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 92413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEHISTEV
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Backbone manufacturerLiu, H & Naismith, JH, University of St Andrews
- Backbone size w/o insert (bp) 5364
- Total vector size (bp) 6247
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsprotein expression in (DE3) strains
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehQPRTase
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Alt namehQPRT
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SpeciesH. sapiens (human)
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Insert Size (bp)894
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MutationR161Q
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Entrez GeneQPRT (a.k.a. HEL-S-90n, QPRTase)
- Promoter T7
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Tag
/ Fusion Protein
- TEV protease cleavable 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI/BspHI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byoriginal
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEHIShqprtR161Q was a gift from Huanting Liu & James Naismith (Addgene plasmid # 92413 ; http://n2t.net/addgene:92413 ; RRID:Addgene_92413) -
For your References section:
Structural and kinetic characterization of quinolinate phosphoribosyltransferase (hQPRTase) from homo sapiens. Liu H, Woznica K, Catton G, Crawford A, Botting N, Naismith JH. J Mol Biol. 2007 Oct 26;373(3):755-63. Epub 2007 Aug 24. 10.1016/j.jmb.2007.08.043 PubMed 17868694