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pEHIShqprtK139A
(Plasmid #92403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 92403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEHISTEV
  • Backbone manufacturer
    Liu, H & Naismith, JH, University of St Andrews
  • Backbone size w/o insert (bp) 5364
  • Total vector size (bp) 6247
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression in (DE3) strain.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hQPRTase
  • Alt name
    hQPRT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    894
  • Mutation
    K139A
  • Entrez Gene
    QPRT (a.k.a. HEL-S-90n, QPRTase)
  • Promoter T7
  • Tag / Fusion Protein
    • TEV cleacable 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI/BspHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEHIShqprtK139A was a gift from Huanting Liu & James Naismith (Addgene plasmid # 92403 ; http://n2t.net/addgene:92403 ; RRID:Addgene_92403)

  • For your References section:

    Structural and kinetic characterization of quinolinate phosphoribosyltransferase (hQPRTase) from homo sapiens. Liu H, Woznica K, Catton G, Crawford A, Botting N, Naismith JH. J Mol Biol. 2007 Oct 26;373(3):755-63. Epub 2007 Aug 24. 10.1016/j.jmb.2007.08.043 PubMed 17868694
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