PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
(Plasmid #92400)

• Purpose: Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.
• Depositing Lab: Periklis Pantazis
• Publication: Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371/journal.pone.0032888. Epub 2012 Mar 14.

Backbone

• Vector backbone: pMTB
• Backbone size w/o insert (bp): 6079
• Total vector size (bp): 8358
• Vector type: Zebrafish Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Dendra2
• Species: Synthetic
• Insert Size (bp): 717
• GenBank ID: GU734658.1
• Tag / Fusion Protein:
  • H2B tag (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: SP6 - atttaggtgacactatagaagag
• 3′ sequencing primer: ccttagtcaccgccttcttg

Gene/Insert 2

• Gene/Insert name: Cerulean
• Species: Synthetic
• Insert Size (bp): 717
• GenBank ID: KP666136.1
• Tag / Fusion Protein:
  • membrane tag (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: caggaGAGGGCAGAGGAA
• 3′ sequencing primer: T7 - CCGCTGAGCAATAACTAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry